Bruker timsTOF Study Maps 179,000 Human Plasma Proteoforms

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Bruker Corporation announced a seminal bioRxiv preprint describing a draft atlas of the human plasma proteome at isoform, or proteoform, resolution, conducted by principal investigator Markus Ralser at Charité – Universitätsmedizin Berlin and collaborators. By combining intact protein fractionation with diaPASEF mass spectrometry on Bruker timsTOF mass spectrometry systems, the researchers detected 5,077 plasma protein groups across 179,049 distinct proteoforms, with a high-confidence map comprising 44,083 proteoforms and a median of eleven proteoforms per protein. The study indicates that canonical proteins account only for a fraction of protein diversity in the blood stream and may constitute less than half of total protein content, and the workflow did not use nanoparticle-based enrichment. Dr. Markus Ralser, Einstein Professor of Biochemistry and Head of the Institute of Biochemistry at Charité – Universitätsmedizin Berlin, said the first draft of the plasma proteoform atlas shows that proteoforms are not rare exceptions but a defining feature of plasma biology. The preprint, titled "A draft atlas of the human plasma proteome at isoform resolution," was posted on September 28, 2026, and the draft isoform atlas is intended as a starting point for future proteoform in-depth studies across individuals, physiological states and diseases.

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Bruker's timsTOF mass spectrometry systems enabled a seminal plasma proteoform atlas study, showcasing its technology's capability.

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